B CUBE (TUD) Archives - RTG 3120 Biomolecular Condensates https://dresdencondensates.org/portfolio_cat/bcube/ From Physics to Biological Functions Wed, 04 Mar 2026 10:17:51 +0000 en-US hourly 1 https://wordpress.org/?v=7.0.2 https://dresdencondensates.org/wp-content/uploads/2022/01/cropped-Icon-32x32.jpg B CUBE (TUD) Archives - RTG 3120 Biomolecular Condensates https://dresdencondensates.org/portfolio_cat/bcube/ 32 32 Michael Schlierf https://dresdencondensates.org/portfolio/michael-schlierf/ Wed, 09 Jul 2025 13:26:04 +0000 https://dresdencondensates.org/?post_type=portfolio&p=1344 Conformational Dynamics in Biomolecules

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Portrait from Michael Schlierf
© TUD CMCB, Magdalena Gonciarz

Expertise

The Schlierf group has a strong background in single-molecule biophysics, in particular single-molecule FRET and force spectroscopy method development as well as application to dynamic structural biology questions.  Key research topics include DNA replication, DNA recombination and membrane protein structure and dynamics1-5. In recent years, the group has been involved in defining community standards for data acquisition and analysis6,7, developed large-throughput single-molecule methods8 and discovered increased structural dynamics in polypeptides through chaperone binding9 and mechanical regulation of DNA recombination10. In collaboration with the Alberti and the Jahnel labs, RNA structures and dynamics during protein interactions and in condensates are characterized, elucidating RNA regulatory mechanisms.

References:

  1. Cheng et al. Bacterial initiators form dynamic filaments on single-stranded DNA monomer by monomer. Nucleic Acids Res. 2015;43(1):396-405. https://doi.org/10.1093/nar/gku1284
  2. Grieb et al. Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality. Nucleic Acids Res. 2017;45(18):10555-10563. https://doi.org/10.1093/nar/gkx670
  3. Schlierf et al. Hexameric helicase G40P unwinds DNA in single pair steps. Deindl S, Kuriyan J, Spies M, eds. eLife. 2019;8:e42001. https://doi.org/10.7554/eLife.42001
  4. Krainer et al. A minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR. Commun Biol. 2018;1(1):154. https://doi.org/10.1038/s42003-018-0153-0
  5. Swoboda et al. Enzymatic Oxygen Scavenging for Photostability without pH Drop in Single-Molecule Experiments. ACS Nano. 2012;6(7):6364-6369. https://doi.org/10.1021/nn301895c
  6. Hellenkamp et al. Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study. Nat Methods. 2018;15(9):669-676. https://doi.org/10.1038/s41592-018-0085-0
  7. Agam et al. Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins. Nat Methods. 2023;20(4):523-535. https://doi.org/10.1038/s41592-023-01807-0
  8. Hartmann et al. An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics. Nat Commun. 2023;14(1):6511. https://doi.org/10.1038/s41467-023-42232-3
  9. Chamachi et al. Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates. Proc Natl Acad Sci. 2022;119(9):e2118919119. https://doi.org/10.1073/pnas.2118919119
  10. Vorobevskaia et al. The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex. bioRxiv. Published online January 1, 2024:2024.04.09.588808. https://doi.org/10.1101/2024.04.09.588808

Michael Schlierf Group

Conformational Dynamics in Biomolecules

Discipline: Biophysics

Affiliation: B CUBE (TU Dresden) | Physics of Life (TU Dresden)

Contact: Michael.schlierf [AT] tu-dresden [DOT] de

RTG Project: B1

Current news by this research group

Awards,Simon Alberti Group,Anthony Hyman Group,RTG 3120

Alberti and Hyman among Clarivate’s Highly Cited Researchers in 2025

  RTG 3120 PI's have been among the most highly cited authors in 2025, according to Clarivate. The Hyman and Alberti groups lead the way "in the top 1% by citations for their field(s) and publication year in the Web of Science Core Collection" See the press release by TU Dresden:…

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RTG 3120

New Research Training Group for Biomolecular Condensates in Dresden

The DFG approved a funding application to establish a new Research Training Group (RTG 3120) in Dresden to train PhD students interdisciplinary methods and approaches to study Biomolecular Condensates. Read the press releases for more:…

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