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How To

In this section, you can find condensate-relevant methods utilized by the labs in Dresden.

  • The EMBO course on Methods for Studying Phase Separation in Biology was held in March 2019 in the MPI-CBG, Dresden. A manual that contains the methods for in vivo and in vitro investigation of the liquid-liquid phase separation phenomenon was developed. Below are the methods published in that manual:
    • Determining the intracellular pH of HeLa cells
    • Determining the in vivo saturation concentration using quantitative fluorescence microscopy
    • Determining the in vitro saturation concentration using fluorescence microscopy
    • Analysis of client partitioning into polymeric coacervates
    • Characterization of protein-based phase separation parameters using temperature-controlled microscopy
    • Measuring material properties of phase separated protein droplets with dual-trap optical tweezers
    • Quantification of molecular dynamics in phase separated protein solutions using fluorescence correlation spectroscopy
    • Fluorescence recovery after photobleaching (FRAP) in protein condensates
  • Standard Operating Procedure (SOP) protocols have been developed in Dresden to standardize methods used in the field. Currently available SOPs are listed below:
    • FUS protein purification
    • PGL3 protein purification
    • XMAP215 protein purification

Get in Touch

Max Planck Institute of
Molecular Cell Biology and Genetics

Pfotenhauerstr 108
01307 Dresden
Germany

almedawar(at)mpi-cbg(dot)de

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https://t.co/52Y6BvAswA - we showcase condensate research done in Dresden, attract talent, & share acquired knowledge & skills to aid progress in the field

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mpicbg MPI-CBG Dresden @mpicbg ·
30 Nov

It was great to welcome Patrick Cramer @CramerLab, President-elect of @maxplanckpress and Simone Schwanitz, Secretary General @maxplanckpress today @mpicbg. Everyone had the chance to ask questions. Thanks for the visit!

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mpicbg MPI-CBG Dresden @mpicbg ·
2 Sep

Research transforming our knowledge of dementia wins science prize. Article in @DeutscheWelle on Anthony Hyman @mpicbg @HymanLab receiving the Körber Prize today. @KoerberScience. https://www.dw.com/en/research-transforming-our-knowledge-of-dementia-wins-science-prize/a-62981083

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agnestothp Agnes Toth-Petroczy @agnestothp ·
3 Sep

Protein Evolution Special Issue in Honor of Dan Tawfik was published yesterday. Congratulations to @RomeroRomeroML, @cedric_landerer and @JonasPoehls for your contributions!
https://onlinelibrary.wiley.com/doi/10.1002/pro.4397

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ddcondensates Dresden Condensates @ddcondensates ·
27 Aug

Drug discovery enters a new arena with biomolecular condensates modulation, #innovation in #Dresden and #Boston

Dewpoint Therapeutics @dewpoint_tx

Our latest perspective in @NatRevDrugDisc discusses how #condensates may be nodes of disease that can be leveraged as drug targets. We share how to convert our knowledge of condensates into a #drugdiscovery platform. Check it out to learn more: https://rdcu.be/cTLfy https://twitter.com/NatRevDrugDisc/status/1559915863083552778

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CD-CODE (CrowDsourcing COndensate Database and Encyclopedia)

Dresden Science Calendar

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