- The EMBO course on Methods for Studying Phase Separation in Biology was held in March 2019 in the MPI-CBG, Dresden. A manual that contains the methods for in vivo and in vitro investigation of the liquid-liquid phase separation phenomenon was developed. Below are the methods published in that manual:
- Determining the intracellular pH of HeLa cells
- Determining the in vivo saturation concentration using quantitative fluorescence microscopy
- Determining the in vitro saturation concentration using fluorescence microscopy
- Analysis of client partitioning into polymeric coacervates
- Characterization of protein-based phase separation parameters using temperature-controlled microscopy
- Measuring material properties of phase separated protein droplets with dual-trap optical tweezers
- Quantification of molecular dynamics in phase separated protein solutions using fluorescence correlation spectroscopy
- Fluorescence recovery after photobleaching (FRAP) in protein condensates
- Standard Operating Procedure (SOP) protocols have been developed in Dresden to standardize methods used in the field. Currently available SOPs are listed below: